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KMID : 1134820220510070679
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 7 p.679 ~ p.687
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Effect of Quercetin on Paclitaxel-Induced Cell Cycle Arrest and Apoptosis
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Kim Tae-Hee
Song Hee-Ju
Lee Sang-Do
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Abstract
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Paclitaxel is an effective chemotherapeutic agent that is cytotoxic not only to cancer cells but also to normal cells. Thus, in order to reduce the side effects, the co-administration of paclitaxel and natural products such as flavonoids has been proposed. To investigate the anticancer effects of co-treatment of paclitaxel (0.5 and 5 nM) with quercetin (25 and 50 μM), we determined the anti-proliferative effects in MDA-MB-453 cells. Decreased cell proliferation was observed in a dose- and time-dependent manner following co-treatment of 0.5 nM paclitaxel with quercetin. In contrast, co-treatment with 5 nM paclitaxel and quercetin reduced the anti-proliferative effects compared to paclitaxel alone treatment. Next, we investigated the effects on apoptosis and the cell cycle. We observed that 0.5 nM paclitaxel with quercetin resulted in an increased number of apoptotic cells, whereas 5 nM paclitaxel with quercetin reduced the number of apoptotic cells. Moreover, the combined treatment of paclitaxel and quercetin increased cell cycle arrest at the G2/M phase in both the 0.5 and 5 nM paclitaxel groups. We then examined cell cycle-related protein expressions to investigate the mechanism of cell cycle regulation. The results showed that co-treatment of paclitaxel with quercetin regulated not only G1-related proteins but also G2/M-related proteins. These results indicate that the combined treatment of paclitaxel with quercetin increases cell cycle disruption via regulation of cell cycle-related proteins and decreases the incidence of apoptosis in MDA-MB-453 cells.
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KEYWORD
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cell cycle, MDA-MB-453, paclitaxel, quercetin
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